Coding

Part:BBa_K3789000:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-23)


SOD1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need to insert the SOD1 gene sequence with a common secretion signal sequence “mating factor alpha prepro-leader (MF alpha)” into The yeast strain FY4. The selection of yeast gene sequence reduces the possibility of rejection of foreign genes



Source

The sequence we used comes from Saccharomyces cerevisiae S288c


References